SignalLab
Interactive experiments in cell and neural signalling
Fluorescence Lifetime Imaging (FLIM) for studying Synaptic Function
Fluorescence Lifetime Imaging (FLIM) adds another dimension to imaging data
In imaging we record the total fluorescence at a location (as seen in the
2D
heatmap).
FLIM records the time each photon arrives at the detector. Adding a 3rd dimension to segment our data
Indicators
Oregon Green BAPTA-1 ```
Cal-590
iGluSnFR
Normalisation
Arbitrary Units
ΔF (F - F₀)
ΔF/F₀
F₀ Start (s)
F₀ End (s)
NTC & [Ca²⁺] Calculation
Fit Start (ns)
Starting point to seek FLIM peak
Integration Duration (ns)
Integration period after detected peak
Background Threshold
Min photon count threshold
Auto-calculate on selection
Calculate NTC & [Ca²⁺]
NTC Value:
-
[Ca²⁺]:
-
nM
Line Scan Heatmap:
Click-drag
to see lifetime data
Loading image...
Total Fluorescence Over Time
Lifetime Plot: drag on heatmap to populate
In Vitro Calibration Curve
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