Fluorescence Lifetime Imaging (FLIM) for studying Synaptic Function
Fluorescence Lifetime Imaging (FLIM) adds another dimension to imaging data
In imaging we record the total fluorescence at a location (as seen in the 2D heatmap).
FLIM records the time each photon arrives at the detector. Adding a 3rd dimension to segment our data
Line Scan Heatmap: Click-drag to see lifetime data
Total Fluorescence Over Time
Photon Decay Curve